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Registro Completo |
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Biblioteca(s): |
Embrapa Agrobiologia; Embrapa Soja; Embrapa Tabuleiros Costeiros. |
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Data corrente: |
27/06/1995 |
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Data da última atualização: |
09/06/2006 |
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Autoria: |
McGONIGLE, T. P.; MILLER, M. H.; EVANS, D. G.; FAIRCHILD, G. L.; SWAN, J. A. |
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Afiliação: |
University of Gouelph, Guelph, Ontario N1G 2W1, Canada. |
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Título: |
A new method which gives an objective measure of colonization of roots by vesicular-arbuscular mycorrhizal fungi. |
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Ano de publicação: |
1990 |
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Fonte/Imprenta: |
New Phytology, v.115, p.495-501, 1990. |
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Idioma: |
Inglês |
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Conteúdo: |
Previously described methods to quantify the poprortion of root length colonized by vesicular-arbuscular(VA) mycorrhizal fungi are reviewed. It is argued that these methods give observer-dependent measures of colonization which cannot be used to compare, quantitatively, roots examined by diferents researchers. A modified method is described here to estimate VA mycorrhizal colonization on an objective scale of measurement, involving inspection of intersections between the microscope eyepiece crosshair and roots at magnification x200; it is referred to as the magnified intersections method. Whether the vertical eyepiece crosshair crosses one more arbusculesis noted at each intersection. The estimate of colonization is the proportion of roots length containing arbuscules, called the arbuscular colonization (AC). The magnified intersections method also determines the proportion of root length containing vesicles, the vesicular colonization (VC), and the proportion of roots length containing hyphae, the hyphal colonization (HC). However, VC and HC should be interpreted with caution because vesicles and hyphae, unlike arbuscules, can be produced in roots by non-mycorrhizal fungi. |
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Palavras-Chave: |
Arbuscules; Colonization of roots; Fractional infection; Fungo micorriza; Fungo micorrizico; Magnified infection; Magnified intersections; Method; MICORRIZA/VA; Percent infection. |
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Thesagro: |
Colonização; Infecção; Método; Micorriza; Raiz. |
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Thesaurus Nal: |
mycorrhizal fungi. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02182naa a2200361 a 4500
001 1458814
005 2006-06-09
008 1990 bl --- 0-- u #d
100 1 $aMcGONIGLE, T. P.
245 $aA new method which gives an objective measure of colonization of roots by vesicular-arbuscular mycorrhizal fungi.
260 $c1990
520 $aPreviously described methods to quantify the poprortion of root length colonized by vesicular-arbuscular(VA) mycorrhizal fungi are reviewed. It is argued that these methods give observer-dependent measures of colonization which cannot be used to compare, quantitatively, roots examined by diferents researchers. A modified method is described here to estimate VA mycorrhizal colonization on an objective scale of measurement, involving inspection of intersections between the microscope eyepiece crosshair and roots at magnification x200; it is referred to as the magnified intersections method. Whether the vertical eyepiece crosshair crosses one more arbusculesis noted at each intersection. The estimate of colonization is the proportion of roots length containing arbuscules, called the arbuscular colonization (AC). The magnified intersections method also determines the proportion of root length containing vesicles, the vesicular colonization (VC), and the proportion of roots length containing hyphae, the hyphal colonization (HC). However, VC and HC should be interpreted with caution because vesicles and hyphae, unlike arbuscules, can be produced in roots by non-mycorrhizal fungi.
650 $amycorrhizal fungi
650 $aColonização
650 $aInfecção
650 $aMétodo
650 $aMicorriza
650 $aRaiz
653 $aArbuscules
653 $aColonization of roots
653 $aFractional infection
653 $aFungo micorriza
653 $aFungo micorrizico
653 $aMagnified infection
653 $aMagnified intersections
653 $aMethod
653 $aMICORRIZA/VA
653 $aPercent infection
700 1 $aMILLER, M. H.
700 1 $aEVANS, D. G.
700 1 $aFAIRCHILD, G. L.
700 1 $aSWAN, J. A.
773 $tNew Phytology$gv.115, p.495-501, 1990.
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Registro original: |
Embrapa Soja (CNPSO) |
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